Terminator

Part:BBa_K404108:Design

Designed by: Freiburg Bioware 2010   Group: iGEM10_Freiburg_Bioware   (2010-08-28)

hGH terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 387


Design Notes

For designing the BioBrick part, the iGEM team Freiburg used primers in the RFC10 standard and performed a PCR. The PCR product was digested using XbaI and PstI followed by ligation into the iGEM standard plasmid pSB1C3.

Source

The plasmid was PCR amplified using the vector pAAV_MCS as DNA template.

References

Danckwardt, S., Hentze, M. W., & Kulozik, A. E. (2008). 3' end mRNA processing: molecular mechanisms and implications for health and disease. The EMBO journal, 27(3), 482-98. doi: 10.1038/sj.emboj.7601932.
Millevoi, S., Loulergue, C., Dettwiler, S., Karaa, S. Z., Keller, W., Antoniou, M., et al. (2006). An interaction between U2AF 65 and CF I(m) links the splicing and 3' end processing machineries. The EMBO journal, 25(20), 4854-64. doi: 10.1038/sj.emboj.7601331.